The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19

Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf

The extinction coefficient can be defined as the amount of light that is absorbed by a particular compound at a certain wavelength [186] [187] . Surface glycoprotein had the highest predicted extinction co-efficient of 148960 M -1 cm -1 . The aliphatic index of a protein corresponds to the relative volume occupied by the aliphatic amino acids in the side chains of the protein, for example: alanine, valine etc. [188] [189] . Surface glycoprotein also had the highest predicted aliphatic index among the two proteins (84.67). For this reason, surface glycoprotein had greater amount of aliphatic amino acids in its side chain than the nucleocapsid phosphoprotein. The grand average of hydropathicity value (GRAVY) for a protein is calculated as the sum of hydropathy values of all the amino acids of the protein, divided by the number of residues in its sequence [190] . Surface [191] - [195] . The possible T-cell and Bcell epitopes of the selected proteins were determined by the IEDB (https://www.iedb.org/) server.