The SARS-CoV-2 receptor-binding domain elicits a potent neutralizing response without antibody-dependent enhancement

Brian D Quinlan; Huihui Mou; Lizhou Zhang; Yan Gao; Wenhui He; Amrita Ojha; Mark S Parcells; Guangxiang Luo; Wenhui Li; Guocai Zhong; Hyeryun Choe; Michael Farzan

Thus anti-RBD anti-serum did not promote ADE at serum dilutions and under conditions in which . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.10.036418 doi: bioRxiv preprint ZIKV ADE could be easily observed. Finally we confirmed the results shown in Figure 3A and B using K562 cells that endogenously express FcγRII (Chiofalo et al., 1988) . Again, day-40 sera did not enhance entry SARS-PV entry, but rat anti-ZIKV antisera again robustly promoted ZIKV-VLP infection ( Figure 3E and 3F). Collectively the data of Figure 3 suggest that antibodies elicited by an RBD-based vaccine are likely to neutralize more efficiently than they would mediate ADE.