Selected article for: "BamH1 site and expression vector"
Author: David N. Frick; Rajdeep S. Virdi; Nemanja Vuksanovic; Narayan Dahal; Nicholas R Silvaggi
Title: Variable Macro X Domain of SARS-CoV-2 Retains the Ability to Bind ADP-ribose
  • Document date: 2020_4_2
    • ID: 02q9y011_14
    Snippet: Gene Synthesis-To facilitate comparison between SARS-CoV-1 and SARS-CoV-2, a protein expression vector was generated similar to the one used by Eglott et al. 9 To this end, a codon optimized open reading frame was synthesized by GenSript (Piscataway, NJ) that encodes the Macro X domain with an N-terminal TEVcleavage site flanked by NheI and BamH1 restriction sites. This open reading frame was cloned into pET21b to give plasmid pET21-COVID-MacroX......
    Document: Gene Synthesis-To facilitate comparison between SARS-CoV-1 and SARS-CoV-2, a protein expression vector was generated similar to the one used by Eglott et al. 9 To this end, a codon optimized open reading frame was synthesized by GenSript (Piscataway, NJ) that encodes the Macro X domain with an N-terminal TEVcleavage site flanked by NheI and BamH1 restriction sites. This open reading frame was cloned into pET21b to give plasmid pET21-COVID-MacroX. The pET11-COVID-MacroX plasmid was used to transform BL21(DE3) cells.

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