Sensitive one-step isothermal detection of pathogen-derived RNAs

Chang Ha Woo; Sungho Jang; Giyoung Shin; Gyoo Yeol Jung; Jeong Wook Lee

RNAs. The samples were then diluted and added to SENSR reaction to investigate the 218 specificity and sensitivity (Fig. 5a ). We observed a significant difference in fluorescence 219 intensity between MRSA and MSSA (Fig. 5b) . The RNA sample from only 2 CFU per 100 220 μL reaction of MRSA, not MSSA, was clearly detected by SENSR, thereby indicating its 221 high sensitivity and specificity even with samples of the living pathogen. Finally, the 222 performance of SENSR was further validated using samples prepared in human serum (Fig. 223 5c). The sensitivity and specificity of SENSR were unaffected by the presence of human 224 serum (Fig. 5d) , indicating the suitability of SENSR in practical applications. 225 226 Dual target detection using orthogonal SENSR probes 227 All rights reserved. No reuse allowed without permission. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.