Author: Timothy A. Dinh; Ramja Sritharan; F. Donelson Smith; Adam B. Francisco; Rosanna K. Ma; Rodica P. Bunaciu; Matt Kanke; Charles G. Danko; Andrew P. Massa; John D. Scott; Praveen Sethupathy
Title: Hotspots of aberrant enhancer activity in fibrolamellar carcinoma reveal molecular mechanisms of oncogenesis and intrinsic drug resistance Document date: 2020_1_18
ID: bf4qpsy7_76
Snippet: Total RNA was isolated using the Total RNA Purification Kit (Norgen Biotek) per manufacturer's instructions. Reverse transcription was performed using the High Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific). Gene expression was quantified with TaqMan Gene Expression Assays (Thermo Fisher Scientific) on a CFX96 Touch Real-Time System (Bio-Rad). RNA expression levels were normalized to RPS9. The TaqMan assays used are provided in Table S8 ......
Document: Total RNA was isolated using the Total RNA Purification Kit (Norgen Biotek) per manufacturer's instructions. Reverse transcription was performed using the High Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific). Gene expression was quantified with TaqMan Gene Expression Assays (Thermo Fisher Scientific) on a CFX96 Touch Real-Time System (Bio-Rad). RNA expression levels were normalized to RPS9. The TaqMan assays used are provided in Table S8 .
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