Selected article for: "real time and Trizol reagent"
Author: Chengcai Lai; Lihui Liu; Qinghua Liu; Sijie Cheng; Keyu Wang; Lingna Zhao; Min Xia; Cheng Wang; Hongjing Gu; Yueqiang Duan; Zhongpeng Zhao; Lili Zhang; Ziyang Liu; Jianjun Luo; Jianxun Song; Penghui Yang; Runsheng Chen; Xiliang Wang
Title: Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a
  • Document date: 2019_4_30
    • ID: 0p8z25c1_6
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/623132 doi: bioRxiv preprint Quantitative real-time PCR 504 Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen). cDNA was 505 generated by reverse transcription with commercial PrimeScript RT Master Mix (Takara). 506 Primer pairs (Table S12) For northern blotting, total RNA was isolated from A549 cells usi.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/623132 doi: bioRxiv preprint Quantitative real-time PCR 504 Total RNA was extracted from cultured cells using TRIzol reagent (Invitrogen). cDNA was 505 generated by reverse transcription with commercial PrimeScript RT Master Mix (Takara). 506 Primer pairs (Table S12) For northern blotting, total RNA was isolated from A549 cells using TRIzol reagent. Probes 523 (Custom LNA mRNA Detection) were designed and synthesized by Exiqon and were also 524 All rights reserved. No reuse allowed without permission.

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