Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_16
Snippet: Two and half microliters of reverse transcriptase reaction (obtained from a reaction mixture before its incubation with glutathione Sepharose binding a GST-eIF4E fusion protein; see above) was subjected to Real-Time PCR amplification (5 min at 94°C; then 55 cycles of 20 sec at 94°C, 30 sec at 50°C, and 45 sec at 72°C; and finally, 4 min at 72°C) using an iCycler iQ Real-Time PCR Detection System (BioRad) and SYBR Green Master Mix (BioRad)......
Document: Two and half microliters of reverse transcriptase reaction (obtained from a reaction mixture before its incubation with glutathione Sepharose binding a GST-eIF4E fusion protein; see above) was subjected to Real-Time PCR amplification (5 min at 94°C; then 55 cycles of 20 sec at 94°C, 30 sec at 50°C, and 45 sec at 72°C; and finally, 4 min at 72°C) using an iCycler iQ Real-Time PCR Detection System (BioRad) and SYBR Green Master Mix (BioRad).
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