Author: Andrew C Nelson; Benjamin Auch; Matthew Schomaker; Daryl M Gohl; Patrick Grady; Darrell Johnson; Robyn Kincaid; Kylene E Karnuth; Jerry Daniel; Jessica K Fiege; Elizabeth J Fay; Tyler Bold; Ryan A Langlois; Kenneth B Beckman; Sophia Yohe
Title: Analytical Validation of a COVID-19 qRT-PCR Detection Assay Using a 384-well Format and Three Extraction Methods Document date: 2020_4_5
ID: ec3egn8o_33
Snippet: . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022186 doi: bioRxiv preprint n/a n/a 4% NEG2 n/a n/a 14% Figure 1 : Representative amplification curves of known negative samples. Exponential amplification curves for the RP internal control target are shown in red. Non-specific, nonexponential curves for.....
Document: . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.02.022186 doi: bioRxiv preprint n/a n/a 4% NEG2 n/a n/a 14% Figure 1 : Representative amplification curves of known negative samples. Exponential amplification curves for the RP internal control target are shown in red. Non-specific, nonexponential curves for either N1 or N2 targets are shown in blue. ΔRn threshold was set at 0.5 to protect against potential false positive calls.
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