Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni

Anastassia K. Pogoutse; Trevor F. Moraes

HsTbpA2 is phase variable, it is possible that HsTbpA2 was not expressed in the bacteria used in 272 the whole-cell binding assays of the previously published study, which means that TbpA 273 specificity may have had a larger relative effect on the results. It is worth noting that a previous 274 study that identified TbpA2 in another strain of H. somni also suggested that H. somni has two 275 sets of receptors, one specific for bovine transferrin and another that bound bovine, ovine, and 276 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/730739 doi: bioRxiv preprint caprine transferrins (16). However, this paper does not conclusively determine which Tbps are 277 responsible for each binding pattern. 278