Selected article for: "probe primer and specific amplification"
Author: Chantal B.F. Vogels; Anderson F. Brito; Anne Louise Wyllie; Joseph R Fauver; Isabel M. Ott; Chaney C. Kalinich; Mary E. Petrone; Marie-Louise Landry; Ellen F. Foxman; Nathan D. Grubaugh
Title: Analytical sensitivity and efficiency comparisons of SARS-COV-2 qRT-PCR assays
  • Document date: 2020_4_1
    • ID: 6mdimxnk_14
    Snippet: All nine primer-probe sets have a similar lower detection limit of 10 2 SARS-CoV-2 genome equivalents/μL. We determined the lower detection limit of nine primer-probe sets as well as the human RNase P control for mock samples (RNA extracted from nasopharyngeal swabs collected in 2017) spiked with known concentrations of SARS-CoV-2 RNA. We performed 6-8 technical replicates with mock samples without spiking RNA and mock samples spiked with 10 0 -.....
    Document: All nine primer-probe sets have a similar lower detection limit of 10 2 SARS-CoV-2 genome equivalents/μL. We determined the lower detection limit of nine primer-probe sets as well as the human RNase P control for mock samples (RNA extracted from nasopharyngeal swabs collected in 2017) spiked with known concentrations of SARS-CoV-2 RNA. We performed 6-8 technical replicates with mock samples without spiking RNA and mock samples spiked with 10 0 -10 2 genome equivalent/μL of SARS-CoV-2 RNA. For each primer-probe set, we show the range of cycle threshold values obtained with mock samples extracted from SARS-CoV-2-negative nasopharyngeal swabs, which indicates variation in the lower detection limit of each primer-probe set. ND = not detected. Gray-shaded areas = non-specific amplification.

    Search related documents:
    Co phrase search for related documents
    • detection limit and known concentration: 1, 2
    • detection limit and nasopharyngeal swab: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • detection limit and non specific amplification: 1, 2, 3, 4
    • detection limit and primer probe: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • detection limit and primer probe set: 1, 2, 3, 4, 5
    • detection limit and primer probe set detection limit: 1
    • detection limit and SARS nasopharyngeal swab: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • human rnase and nasopharyngeal swab: 1, 2, 3, 4, 5
    • human rnase and primer probe: 1, 2, 3, 4, 5, 6
    • human rnase and primer probe set: 1, 2, 3
    • human rnase and SARS nasopharyngeal swab: 1
    • mock sample and primer probe: 1
    • mock sample and primer probe set: 1
    • nasopharyngeal swab and non specific amplification: 1
    • nasopharyngeal swab and primer probe: 1, 2, 3, 4, 5, 6, 7, 8
    • nasopharyngeal swab and primer probe set: 1, 2
    • nasopharyngeal swab and SARS nasopharyngeal swab: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • non specific amplification and primer probe set: 1
    • non specific amplification and SARS nasopharyngeal swab: 1