Author: Guanmin Jiang; Xiaoshuai Ren; Yan Liu; Hongtao Chen; Wei Liu; Zhaowang Guo; Yaqin Zhang; Chaoqun Chen; Jianhui Zhou; Qiang Xiao; Hong Shan
Title: Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection Document date: 2020_2_27
ID: 65plab8b_15
Snippet: RNA was extracted and tested by real-time RT-PCR with SARS-Cov-2 specific primers and probes according to instruction of Kit. The real-time RT-PCR was carried out in biosafety level 2 facilities at the clinic laboratory. If two targets (RdRp+, E or N +) tested positive by specific RT-PCR, the patients would be considered to be laboratory-confirmed......
Document: RNA was extracted and tested by real-time RT-PCR with SARS-Cov-2 specific primers and probes according to instruction of Kit. The real-time RT-PCR was carried out in biosafety level 2 facilities at the clinic laboratory. If two targets (RdRp+, E or N +) tested positive by specific RT-PCR, the patients would be considered to be laboratory-confirmed.
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