Anti-TGEV Miller Strain Infection Effect of Lactobacillus plantarum Supernatant Based on the JAK-STAT1 Signaling Pathway

Wang, Kai; Ran, Ling; Yan, Tao; Niu, Zheng; Kan, Zifei; Zhang, Yiling; Yang, Yang; Xie, Luyi; Huang, Shilei; Yu, Qiuhan; Wu, Di; Song, Zhenhui

As expected, the protein levels of ZAP, PKR, p-PKR (Figures 8A-D) at 24, and 48 hpi in the Lp-1s group were significantly higher than those in negative control group and TGEV group (ZAP, * P < 0.05 and PKR, * * P < 0.01). There was Figure 8E where the expression of this gene was knocked down using siRNA is significantly lower than the level of unknocked down STAT1 in Figure 5A . After gene silencing STAT1, the protein levels of Lp-1s, Lp-1s pretreatment of IPEC-J2 cells infected with TGEV; TGEV, cells directly infected with TGEV; Con, uninfected cells (normal group). There were significant differences in IFN-β levels between the TGEV group and the Lp-1s group at different time points ( * * P < 0.01). 8E-H) at 12, 24, and 48 hpi in the Lp-1s group were significantly higher than TGEV group (p-STAT1/STAT1, * P < 0.05; ZAP, * P < 0.05; and p-PKR/PKR, * P < 0.05). The results showed that Lp-1s could There was no significant difference between the two groups (P > 0.05) at 12 h; however, the difference was extremely significant at 24 and 48 h ( * * P < 0.01). Lp-1s, Lp-1s pretreatment of IPEC-J2 cells infected with TGEV; TGEV, cells directly infected with TGEV. showed that the level of phosphorylated STAT1 was higher in the Lp-1s group than in the TGEV group after 12 h (P > 0.05); after 24 h of Lp-1s treatment, the level of phosphorylated STAT1 in the Lp-1s group was significantly higher than that in the TGEV group ( * * P < 0.01); after 48 h of Lp-1s treatment, the level of phosphorylated STAT1 in the Lp-1s group was significantly higher than that in the TGEV group ( * P < 0.05). The results showed that treatment of TGEV-infected IPEC-J2 cells with Lp-1s (24-48 h), induced increased of levels of phosphorylated intracellular STAT1. The nucleus was identified using Zen blue software and the intensity of red fluorescence emitted by Cy3-labeled p-STAT1 was analyzed. The statistical results showed that the fluorescence intensity of Cy3 in IPEC-J2 cells treated with Lp-1s was significantly higher than that in the TGEV group after 12 h ( * P < 0.05). In the late stage of TGEV infection (24-48 h), the fluorescence signal intensity of p-STAT1 in the nucleus of this group was significantly higher than that in TGEV group ( * * P < 0.01).