Author: Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua
Title: Antibody Engineering for Pursuing a Healthier Future Document date: 2017_3_28
ID: 0fegsm1v_87
Snippet: Various types of phage libraries are used for the screening of specific recombinant proteins/antibodies in relation to antigen specificity: (1) construction of specific high-affinity antibody library for specific antigens by the use of immunized animals with a dissociation constant in the nanomolar range, (2) a singlepot (general) non-specific library produced against antigens, and (3) construction of secondary mutant antibody phage libraries for.....
Document: Various types of phage libraries are used for the screening of specific recombinant proteins/antibodies in relation to antigen specificity: (1) construction of specific high-affinity antibody library for specific antigens by the use of immunized animals with a dissociation constant in the nanomolar range, (2) a singlepot (general) non-specific library produced against antigens, and (3) construction of secondary mutant antibody phage libraries for the screening of antibodies with high specificity (Hust and Dubel, 2004) . The development of an antibody library from immunized animals has become obsolete because of the construction of diverse universal single-pot libraries for the isolation of numerous antigens. PDT has many applications in biotechnology, production of recombinant multifunctional antibodies, cancer, immunotherapeutics, and the enhanced validity of protein fragments (Yau et al., 2003) .
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