Author: Van Nguyen, Dung; Van Nguyen, Cuong; Bonsall, David; Ngo, Tue Tri; Carrique-Mas, Juan; Pham, Anh Hong; Bryant, Juliet E.; Thwaites, Guy; Baker, Stephen; Woolhouse, Mark; Simmonds, Peter
Title: Detection and Characterization of Homologues of Human Hepatitis Viruses and Pegiviruses in Rodents and Bats in Vietnam Document date: 2018_2_28
ID: 0jtfc271_20
Snippet: Two real-time PCR primer sets (Table S5 ) in the 5 untranslated region of bamboo rat hepaciviruses and the screening fragment of other rat hepaciviruses were designed using sequences available from this study. The targeted regions were amplified from positive samples and cloned into pGEM-T Easy Vector (Promega, Southampton, UK) for in vitro transcription, as previously described [30] . The obtained RNA transcripts were used to generate standard c.....
Document: Two real-time PCR primer sets (Table S5 ) in the 5 untranslated region of bamboo rat hepaciviruses and the screening fragment of other rat hepaciviruses were designed using sequences available from this study. The targeted regions were amplified from positive samples and cloned into pGEM-T Easy Vector (Promega, Southampton, UK) for in vitro transcription, as previously described [30] . The obtained RNA transcripts were used to generate standard curves of the real-time PCR assays for measurement of rodent hepacivirus RNA titers using SuperScript III reverse transcriptase (Invitrogen, Paisley, UK) and PowerUp SYBR Green master mix (Thermo Fisher Scientific, Northumberland, UK).
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