Selected article for: "Ct method and pcr system"
Author: Cong, Wei; Zhang, Xiao-Xuan; He, Jun-Jun; Li, Fa-Cai; Elsheikha, Hany M.; Zhu, Xing-Quan
Title: Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection
  • Document date: 2017_3_10
    • ID: 05tshufp_36
    Snippet: The stem-loop quantitative reverse transcription PCR was used to validate the results of miRNA expression analysis as described previously [21, 62] . Stem-loop RT-PCR was performed on ABI PRISM® 7500 Sequence Detection System using SYBR Green qPCRSuperMix according to the manufacturer's protocol (Invitrogen). All qRT-PCR reactions were performed in three replicates. Gene expressions were calculated by 2 −ΔΔCT relative expression method as pr.....
    Document: The stem-loop quantitative reverse transcription PCR was used to validate the results of miRNA expression analysis as described previously [21, 62] . Stem-loop RT-PCR was performed on ABI PRISM® 7500 Sequence Detection System using SYBR Green qPCRSuperMix according to the manufacturer's protocol (Invitrogen). All qRT-PCR reactions were performed in three replicates. Gene expressions were calculated by 2 −ΔΔCT relative expression method as previously described [63] . snRNA U6 was used as normalization control in qRT-PCR.

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