Selected article for: "gradient centrifugation and peripheral blood"
Author: Funaro, Ada; Gribaudo, Giorgio; Luganini, Anna; Ortolan, Erika; Lo Buono, Nicola; Vicenzi, Elisa; Cassetta, Luca; Landolfo, Santo; Buick, Richard; Falciola, Luca; Murphy, Marianne; Garotta, Gianni; Malavasi, Fabio
Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells
  • Document date: 2008_11_12
    • ID: 1u2fkwx3_26
    Snippet: After informed consent was obtained, peripheral blood (8-10 ml) was collected from healthy blood donors or infectious disease patients. Serum was screened for reactivity with HCMV glycoproteins and for HCMV neutralizing activity. Peripheral blood mononuclear cells (PBMCs) were purified from heparinized peripheral blood by density gradient centrifugation on Ficoll/Hypaque (Amersham Pharmacia, Milan, Italy)......
    Document: After informed consent was obtained, peripheral blood (8-10 ml) was collected from healthy blood donors or infectious disease patients. Serum was screened for reactivity with HCMV glycoproteins and for HCMV neutralizing activity. Peripheral blood mononuclear cells (PBMCs) were purified from heparinized peripheral blood by density gradient centrifugation on Ficoll/Hypaque (Amersham Pharmacia, Milan, Italy).

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