Author: van der Schaar, H. M.; Melia, C. E.; van Bruggen, J. A. C.; Strating, J. R. P. M.; van Geenen, M. E. D.; Koster, A. J.; Bárcena, M.; van Kuppeveld, F. J. M.
Title: Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein Document date: 2016_7_6
ID: 1aptufp6_19
Snippet: indicating that binding of GFP(S1-10) to 3A(S11aa2) does not impede replication ( Fig. 2A and B ). Both infectious virus production and viral RNA levels of the tagged CVB3 were delayed compared to those of wt CVB3 (Fig. 2B ). This delay in replication has also been observed for recombinant polioviruses that encode 3A proteins with small epitope tags (32) . Together, these findings showed that replication of CVB3-3A(S11aa2) is similar to other ent.....
Document: indicating that binding of GFP(S1-10) to 3A(S11aa2) does not impede replication ( Fig. 2A and B ). Both infectious virus production and viral RNA levels of the tagged CVB3 were delayed compared to those of wt CVB3 (Fig. 2B ). This delay in replication has also been observed for recombinant polioviruses that encode 3A proteins with small epitope tags (32) . Together, these findings showed that replication of CVB3-3A(S11aa2) is similar to other enteroviruses that encode small tags in a viral protein.
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