Author: Jordana-Lluch, Elena; Giménez, Montserrat; Quesada, M. Dolores; Ausina, Vicente; Martró, Elisa
Title: Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry Document date: 2014_4_9
ID: 05zahl5n_31
Snippet: Fungi are causative agents of infections, but due to the slow growth of these microorganisms, identification by culture is often impractical. Recently, a new assay for the PCR/ESI-MS systems has been validated for detection of Aspergillus spp., Candida spp., Pneumocystis spp., Cryptococcus spp., Mucor spp., and Rhizopus spp. [57] . Concordance rates between PCR/ESI-MS and phenotypic identification and sequencing were 89.7% at the genus level and .....
Document: Fungi are causative agents of infections, but due to the slow growth of these microorganisms, identification by culture is often impractical. Recently, a new assay for the PCR/ESI-MS systems has been validated for detection of Aspergillus spp., Candida spp., Pneumocystis spp., Cryptococcus spp., Mucor spp., and Rhizopus spp. [57] . Concordance rates between PCR/ESI-MS and phenotypic identification and sequencing were 89.7% at the genus level and 87.4% at the species level. Although most of the experiments in this study were performed with reference strains and clinical isolates, detection of Aspergillus terreus directly from a culturenegative bronchioalveolar lavage was demonstrated [58] .
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