Author: Priolo, Vito; Martínez-Orellana, Pamela; Pennisi, Maria Grazia; Masucci, Marisa; Prandi, David; Ippolito, Dorotea; Bruno, Federica; Castelli, Germano; Solano-Gallego, Laia
Title: Leishmania infantum-specific IFN-? production in stimulated blood from cats living in areas where canine leishmaniosis is endemic Document date: 2019_3_26
ID: 04ljoezz_15
Snippet: Total DNA was extracted from EDTA blood using the DNA Gene extraction kit (Sigma Aldrich, Saint Louis, MO, USA) following the manufacturer's instructions with some modifications. Forty microliters of proteinase K solution were added to all samples. Four hundred microliters of whole blood were used for all the samples. Blood from a clinically healthy non-infected cat was used as a control for DNA contamination in every DNA extraction performed. Th.....
Document: Total DNA was extracted from EDTA blood using the DNA Gene extraction kit (Sigma Aldrich, Saint Louis, MO, USA) following the manufacturer's instructions with some modifications. Forty microliters of proteinase K solution were added to all samples. Four hundred microliters of whole blood were used for all the samples. Blood from a clinically healthy non-infected cat was used as a control for DNA contamination in every DNA extraction performed. The real-time polymerase chain reaction (RT-PCR) was carried out in a CFX96 Real-time System (Bio-Rad Laboratories s.r.l., Hercules, CA, USA) using TaqMan Master Mix (Applied Biosystems by Ther-moFisher, Waltham, MA, USA) and performed as previously described [22] .
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