Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

Canine parvovirus (CPV), a member of the Parvovirus genus, is one of the most common etiological agents that cause severe gastroenteritis in puppies of mainly 6-20 weeks old, especially unvaccinated puppies or those with poor maternal protection through passive immunity [3, 4] . Parvovirus replicates mainly in intestinal crypts and characterized by being highly contagious. CPV is a variant of the feline panleukopenia virus (FPV) with the new nomenclature of canine parvovirus type 2 (CPV-2) and differs genetically and antigenically from the canine minute virus, which designed as CPV-1 [5] . CPV-2 is responsible for hemorrhagic gastroenteritis with high rates of mortality and morbidity and became widespread since 1978 [6] . From the outbreak of CPV-2, three new antigenic variants were characterized and termed as CPV-2a, CPV-2b and CPV-2c [7] . However,the clinical diagnosis of CPV-2 infection is indecisive, since several other pathogens may cause diarrhoea in dogs [8] . Therefore, early accurate diagnosis of CPV-2 infection is quintessential, so that the infected dogs can receive timely and effective treatment to reduce morbidity and mortality.