Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

In recent years, magnetic separation has become an interesting and useful tool for bioassays because the magnetic beads enable the isolation or extraction of target molecule or substance under the action of an external magnetic field [23, 24] . Due to the good biocompatibility and adequate functional groups for chemical fixation, magnetic beads modified with various recognition elements can be used for specific bioaffinity capture of different molecules. They are employed not only for magnetic separation of support from the reaction mixture but also as the solid adsorbent [25] . Therefore, magnetic beads have been applied to other areas such as immobilization of proteins and enzymes [26] , bioseparation [27] , immunoassay [28] , drug delivery [29] and biosensors [30] .