Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_10
Snippet: The polymerase chain reaction (PCR) primers were especially designed from consensus genome regions of VP2 gene according to previous study [32] . The biotin-labeled forward primer was 5'-Biotin-AATGTACCACCAGT TTATC-3′ and the digoxigenin-labeled reverse primer was 5′-digoxigenin-TGGGAGGCTCTTAGTTTAG-3′. The primers were assessed using NUPACK web software (http://www.nupack.org/) and their specificity was further verified by BLAST tool (http:.....
Document: The polymerase chain reaction (PCR) primers were especially designed from consensus genome regions of VP2 gene according to previous study [32] . The biotin-labeled forward primer was 5'-Biotin-AATGTACCACCAGT TTATC-3′ and the digoxigenin-labeled reverse primer was 5′-digoxigenin-TGGGAGGCTCTTAGTTTAG-3′. The primers were assessed using NUPACK web software (http://www.nupack.org/) and their specificity was further verified by BLAST tool (http://blast.ncbi.nlm.nih.gov/Blast .cgi). The upstream and downstream primers are located in the conserved region of the VP2 gene and reasonably avoid the mutation sites [33] . Primers were synthesized by GENEWIZ (GENEWIZ, Suzhou, China).
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