Author: Delvecchio, Rodrigo; Higa, Luiza M.; Pezzuto, Paula; Valadão, Ana Luiza; Garcez, Patrícia P.; Monteiro, Fábio L.; Loiola, Erick C.; Dias, André A.; Silva, Fábio J. M.; Aliota, Matthew T.; Caine, Elizabeth A.; Osorio, Jorge E.; Bellio, Maria; O’Connor, David H.; Rehen, Stevens; de Aguiar, Renato Santana; Savarino, Andrea; Campanati, Loraine; Tanuri, Amilcar
Title: Chloroquine, an Endocytosis Blocking Agent, Inhibits Zika Virus Infection in Different Cell Models Document date: 2016_11_29
ID: 01s21vh0_19
Snippet: Vero cells were inoculated with ZIKV MR 766 at an MOI of 10 for 1 h at 4 • C. Cells were washed three times with cold PBS to remove unbound virus and treated with 50 µM chloroquine that was added at different time points: 0, 0.5, 3, 12, and 24 h post-infection. Conditioned media were collected at 30 h post-infection to analyze the production of virus particles through viral RNA content or the amount of infectious virus particles. Viral RNA was.....
Document: Vero cells were inoculated with ZIKV MR 766 at an MOI of 10 for 1 h at 4 • C. Cells were washed three times with cold PBS to remove unbound virus and treated with 50 µM chloroquine that was added at different time points: 0, 0.5, 3, 12, and 24 h post-infection. Conditioned media were collected at 30 h post-infection to analyze the production of virus particles through viral RNA content or the amount of infectious virus particles. Viral RNA was extracted and quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed. The titer of infectious virus particles was determined by plaque assay.
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