Selected article for: "ethidium bromide and PCR reaction"
Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2
  • Document date: 2019_1_17
    • ID: 1c5ug64m_30
    Snippet: Being visual inspection not applicable, to provide a faster way to quantify the PCR results rather than gel electrophoresis, the first polymerase chain reaction combined with fluorescent lateral flow immunoassay (PCR-LFIA) based on magnetic beads for rapid detection of CPV-2 has been successfully developed in this study. The lateral flow immunoassay used nanoparticles as signal amplifier, which ensures the sensitivity and accuracy of this assay. .....
    Document: Being visual inspection not applicable, to provide a faster way to quantify the PCR results rather than gel electrophoresis, the first polymerase chain reaction combined with fluorescent lateral flow immunoassay (PCR-LFIA) based on magnetic beads for rapid detection of CPV-2 has been successfully developed in this study. The lateral flow immunoassay used nanoparticles as signal amplifier, which ensures the sensitivity and accuracy of this assay. The optimized PCR-LFIA only needs about 80 min from PCR step, which is faster than PCR-GE and free of expensive equipment and toxic reagents. Due to the PCR-LFIA does not need gel electrophoresis, it is time-saving and carcinogenic ethidium bromide does not have to be used. The PCR products can be easily detected by LFIA based on magnetic beads purification, which can reduce the total time for detection of CPV-2. Furthermore, the accuracy and specificity of the PCR-LFIA will be greatly improved because magnetic beads for PCR products purification are employed. It can effectively remove the pollution of oligonucleotides, primer dimers, salts and proteins in PCR products or enzymatic reaction solutions.

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