Author: Cong, Yingying; Kriegenburg, Franziska; de Haan, Cornelis A. M.; Reggiori, Fulvio
Title: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers Document date: 2017_7_18
ID: 15hzah62_29
Snippet: After centrifugation at 135,000 × g for 75 min at 4 °C in a TLS55 rotor (Beckman Coulter, Brea, CA), 11 fractions of 200 µl were collected from the top to the bottom of the gradient. After precipitation by addition of 20 µl of tri-chloroacetic acid (final concentration 10%), proteins were resolved by SDS-PAGE and analyzed by western blot using antibodies against the N protein, GAPDH and the 6xHis tag. Thyroglobulin (669 kDa) and ovalbumin (44.....
Document: After centrifugation at 135,000 × g for 75 min at 4 °C in a TLS55 rotor (Beckman Coulter, Brea, CA), 11 fractions of 200 µl were collected from the top to the bottom of the gradient. After precipitation by addition of 20 µl of tri-chloroacetic acid (final concentration 10%), proteins were resolved by SDS-PAGE and analyzed by western blot using antibodies against the N protein, GAPDH and the 6xHis tag. Thyroglobulin (669 kDa) and ovalbumin (44 kDa) (Bio-Rad, Berkeley, CA) were used as molecular weight protein standards to determine the gradient resolution.
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