Selected article for: "real time and siRNA transfection"
Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p
  • Document date: 2014_8_13
    • ID: 1i6hni9s_23
    Snippet: OFSCs were transfected with the miR-671-5p inhibitor (Dharmacon, Bonn, Germany) at a final concentration of 5 nM using GenMuteâ„¢ siRNA Transfection Reagent (SignaGen Laboratories, Pittsburgh, PA, USA) according to the manufacturer's instructions. The level of miR-671-5p in OFSCs with or without macrophage co-culture was measured by real-time reverse transcription polymerase chain reaction after 6 hours of miR-671-5p inhibitor transfection, and t.....
    Document: OFSCs were transfected with the miR-671-5p inhibitor (Dharmacon, Bonn, Germany) at a final concentration of 5 nM using GenMuteâ„¢ siRNA Transfection Reagent (SignaGen Laboratories, Pittsburgh, PA, USA) according to the manufacturer's instructions. The level of miR-671-5p in OFSCs with or without macrophage co-culture was measured by real-time reverse transcription polymerase chain reaction after 6 hours of miR-671-5p inhibitor transfection, and the putative target gene expressions in OFSCs were evaluated under co-culture experiments.

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