Author: Kouokam, Joseph Calvin; Lasnik, Amanda B.; Palmer, Kenneth E.
Title: Studies in a Murine Model Confirm the Safety of Griffithsin and Advocate Its Further Development as a Microbicide Targeting HIV-1 and Other Enveloped Viruses Document date: 2016_11_17
ID: 096348l5_3
Snippet: To evaluate the effect of GRFT on cytokine release in mPBMCs, cells were treated for 24, 48, and 72 h with 1 or 4 µM GRFT, with culture supernatants collected at each time point. Interleukin (IL) 1 beta (1b), IL 6 (IL-6), IL 10 (IL-10), and tumor necrosis factor alpha (TNF-α) amounts in supernatants were assessed by enzyme linked immunosorbent assay (ELISA) using specific ELISA Ready-SET-Go! kits from eBioscience (San Diego, CA, USA), following.....
Document: To evaluate the effect of GRFT on cytokine release in mPBMCs, cells were treated for 24, 48, and 72 h with 1 or 4 µM GRFT, with culture supernatants collected at each time point. Interleukin (IL) 1 beta (1b), IL 6 (IL-6), IL 10 (IL-10), and tumor necrosis factor alpha (TNF-α) amounts in supernatants were assessed by enzyme linked immunosorbent assay (ELISA) using specific ELISA Ready-SET-Go! kits from eBioscience (San Diego, CA, USA), following the manufacturer's instructions. In these experiments, ConA (0.37 and 1 µM) and phosphate-buffered saline (PBS) were used as positive and negative controls, respectively, as previously described [12] .
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