Selected article for: "amplified product and detection system"
Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p
  • Document date: 2014_8_13
    • ID: 1i6hni9s_19
    Snippet: Total RNAs from OFSCs and macrophages were isolated using Trizol (Invitrogen, Carlsbad, CA, USA). Reverse transcription was performed using MMLV reverse transcriptase (Invitrogen). One microliter of the reverse transcription product was amplified using primer pairs specific for miR-671-5p, IFNγ, TNFα, IL-1α, IL-1β, TGFβ, IL-10, IL-1RA, sTNFR type II, and IDO. GAPDH, 18S, and RUN44 were used as controls for quantitation. Real-time polymerase .....
    Document: Total RNAs from OFSCs and macrophages were isolated using Trizol (Invitrogen, Carlsbad, CA, USA). Reverse transcription was performed using MMLV reverse transcriptase (Invitrogen). One microliter of the reverse transcription product was amplified using primer pairs specific for miR-671-5p, IFNγ, TNFα, IL-1α, IL-1β, TGFβ, IL-10, IL-1RA, sTNFR type II, and IDO. GAPDH, 18S, and RUN44 were used as controls for quantitation. Real-time polymerase chain reactions were conducted in an ABI Prism 7300 Sequence Detection System using SYBR Green PCR core reagents (Applied Biosystems, Foster City, CA, USA). The forward and reverse primers for the amplifications are presented in Table 1 . Primers for miR-671-5p were purchased from Ambion, and miR-671-5p levels were assayed following the manufacturer's protocol.

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