Author: Xing, Yang; Liqi, Zhu; Jian, Lin; Qinghua, Yu; Qian, Yang
Title: Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells Document date: 2017_2_1
ID: 1ljye9pj_23
Snippet: TGEV (SHXB strain) was a kind gift of Jiangsu Provincial Academy of Environmental Science (JAAS), and propagated in ST cells. IPEC-J2 cells were treated with DOX at the concentrations indicated for 24 h. Then those cells were infected with TGEV at MOI of 5 for 1 h at 37 • C. Unattached viruses were removed and the cells were washed 3 times with PBS. The cells were incubated in 2% FBS medium supplemented with DOX at indicted concentrations. The .....
Document: TGEV (SHXB strain) was a kind gift of Jiangsu Provincial Academy of Environmental Science (JAAS), and propagated in ST cells. IPEC-J2 cells were treated with DOX at the concentrations indicated for 24 h. Then those cells were infected with TGEV at MOI of 5 for 1 h at 37 • C. Unattached viruses were removed and the cells were washed 3 times with PBS. The cells were incubated in 2% FBS medium supplemented with DOX at indicted concentrations. The samples were harvested and viral titers were determined by 50% endpoint dilution assays (50% tissue culture infective dose [TCID50]) or viral plaque assay on ST cells.
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