Author: Soto-Rodriguez, Guadalupe; Gonzalez-Barrios, Juan-Antonio; Martinez-Fong, Daniel; Blanco-Alvarez, Victor-Manuel; Eguibar, Jose R.; Ugarte, Araceli; Martinez-Perez, Francisco; Brambila, Eduardo; Millán-Perez Peña, Lourdes; Pazos-Salazar, Nidia-Gary; Torres-Soto, Maricela; Garcia-Robles, Guadalupe; Tomas-Sanchez, Constantino; Leon-Chavez, Bertha Alicia
Title: Analysis of Chemokines and Receptors Expression Profile in the Myelin Mutant Taiep Rat Document date: 2015_3_25
ID: 0nb4laxz_7
Snippet: The total RNA (1.0 g) extracted from 1-to 6-month-old brainstem of SD and taiep rats was quantified with a NanoDrop Spectrophotometer (Thermo Scientific NanoDrop Technologies, Wilmington, DE, USA). Reverse transcription reaction was made with the RT 2 PCR Array First Strand Kit from SABiosciences (Qiagen Company). Real-time PCR was conducted on a 384-well plate for Chemokines and Receptors RT 2 Profiler PCR Array of Rat (PARN-022Z, Qiagen), which.....
Document: The total RNA (1.0 g) extracted from 1-to 6-month-old brainstem of SD and taiep rats was quantified with a NanoDrop Spectrophotometer (Thermo Scientific NanoDrop Technologies, Wilmington, DE, USA). Reverse transcription reaction was made with the RT 2 PCR Array First Strand Kit from SABiosciences (Qiagen Company). Real-time PCR was conducted on a 384-well plate for Chemokines and Receptors RT 2 Profiler PCR Array of Rat (PARN-022Z, Qiagen), which contains a profile for the expression of 84 genes that encode chemokines and their receptors and cytokines. The amplification assays were made using a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) (http://www.sabiosciences .com/PCRArrayPlate.php).
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