Selected article for: "denaturation step and extension step"
Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2
  • Document date: 2019_1_17
    • ID: 1c5ug64m_11
    Snippet: Polymerase chain reaction was set up by adding 10 μL Premix Taq™ (TaKaRa Taq™ Version 2.0) (TaKaRa, Dalian, China), 1 μL PCR primers (10 μM each), 1 μL DNA template and the reaction volume was made up to 20 μL using nuclease free ddH 2 O. PCR amplification was performed with an initial denaturation step of 94°C for 5 min, 35 cycles of denaturation at 94°C for 20 s, annealing at 50°C for 20 s, and elongation at 72°C for 20 s, followed.....
    Document: Polymerase chain reaction was set up by adding 10 μL Premix Taq™ (TaKaRa Taq™ Version 2.0) (TaKaRa, Dalian, China), 1 μL PCR primers (10 μM each), 1 μL DNA template and the reaction volume was made up to 20 μL using nuclease free ddH 2 O. PCR amplification was performed with an initial denaturation step of 94°C for 5 min, 35 cycles of denaturation at 94°C for 20 s, annealing at 50°C for 20 s, and elongation at 72°C for 20 s, followed by a final extension step of 72°C for 8 min. characterised to assess the antigenic type according to previous report [33] .

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