Author: Cooray, Samantha; Jin, Li; Best, Jennifer M
Title: The involvement of survival signaling pathways in rubella-virus induced apoptosis Document date: 2005_1_4
ID: 1i36lsj2_45
Snippet: RK13 cells were grown to confluence in 96-well tissue culture plates at 37°C in 5% CO 2 in air. Cells were treated, in a final volume of 100 µl, with RV and kinase inhibitors as described above. At indicated times p.i., 50 µl of labeling mixture containing XTT (sodium 3'-[1-(phenylaminocarbonyl)-3, 4-tetrazolium]-bis (4-methoxy-6-nitro) and coupling reagent PMS (N-methyl dibenzopyrazine methyl sulphate) (Roche Applied Science, Mannheim, German.....
Document: RK13 cells were grown to confluence in 96-well tissue culture plates at 37°C in 5% CO 2 in air. Cells were treated, in a final volume of 100 µl, with RV and kinase inhibitors as described above. At indicated times p.i., 50 µl of labeling mixture containing XTT (sodium 3'-[1-(phenylaminocarbonyl)-3, 4-tetrazolium]-bis (4-methoxy-6-nitro) and coupling reagent PMS (N-methyl dibenzopyrazine methyl sulphate) (Roche Applied Science, Mannheim, Germany) was added directly to the wells to give final concentrations of 0.3 mg/ml and 2.5 µg/ml respectively. Plates were incubated in a humidified atmosphere (37°C, 5% CO 2 ) for 24 hours. The absorbance of the formazan product was measured at a test wavelength of 450 nm and a reference wavelength of 690 nm.
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