Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Lukashev, Alexander N.; Gmyl, Anatoly; Coutard, Bruno; Adam, Alexander; Ritz, Daniel; Leijten, Lonneke M.; van Riel, Debby; Kallies, Rene; Klose, Stefan M.; Gloza-Rausch, Florian; Binger, Tabea; Annan, Augustina; Adu-Sarkodie, Yaw; Oppong, Samuel; Bourgarel, Mathieu; Rupp, Daniel; Hoffmann, Bernd; Schlegel, Mathias; Kümmerer, Beate M.; Krüger, Detlev H.; Schmidt-Chanasit, Jonas; Setién, Alvaro Aguilar; Cottontail, Veronika M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn; Osterrieder, Klaus; Bartenschlager, Ralf; Matthee, Sonja; Beer, Martin; Kuiken, Thijs; Reusken, Chantal; Leroy, Eric M.; Ulrich, Rainer G.; Drosten, Christian
Title: Evidence for Novel Hepaciviruses in Rodents Document date: 2013_6_20
ID: 1v353uij_11
Snippet: Six nested PCR assays for amplification of hepacivirus RNA and two assays targeting the Flaviviridae sister-genera Flavivirus and Pestivirus were used to ensure broad detection. Highly sensitive HCV-specific assays targeting the X-tail, NS5B and 59-untranslated genomic regions were used in addition (see Supplementary Table S2 for oligonucleotide sequences and reaction conditions). RNA quantification relied on strain-specific real-time RT-PCR ass.....
Document: Six nested PCR assays for amplification of hepacivirus RNA and two assays targeting the Flaviviridae sister-genera Flavivirus and Pestivirus were used to ensure broad detection. Highly sensitive HCV-specific assays targeting the X-tail, NS5B and 59-untranslated genomic regions were used in addition (see Supplementary Table S2 for oligonucleotide sequences and reaction conditions). RNA quantification relied on strain-specific real-time RT-PCR assays and photometrically quantified in vitro RNA transcripts generated as described previously [35] .
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