Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_59
Snippet: RNA preparation, quality analysis and microarray analysis were performed as previously described [93] . Microarrays assessed genome-wide macrophage mRNA expression using the GeneChip Human Exon 1.0 ST Arrays (Affymetrix). Microarray data were assessed by paired t-test and limma statistical analysis using R statistical software (http://www.R-project.org). Data were assessed for quality using a normalized unscaled standard error (NUSE) analysis. Th.....
Document: RNA preparation, quality analysis and microarray analysis were performed as previously described [93] . Microarrays assessed genome-wide macrophage mRNA expression using the GeneChip Human Exon 1.0 ST Arrays (Affymetrix). Microarray data were assessed by paired t-test and limma statistical analysis using R statistical software (http://www.R-project.org). Data were assessed for quality using a normalized unscaled standard error (NUSE) analysis. The method of statistical analysis was a paired t-test for each array. Limma analysis was performed to compare both macrophage arrays [94] . A 2-fold change in gene expression and p value of 0.01 was considered significant. Gene pathway analysis was conducted using GeneGo/ Metacore version 6.19 (Thompson Reuters).
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