Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes Document date: 2014_8_3
ID: 1e44usl6_20
Snippet: Nontarget Advances in Bioinformatics Table 4 : Number of nontarget amplicons predicted in a multiplex reaction of tiled primers for 10 kb amplicons. As in Table 3 , but for the multiplexes of the 10 kb-amplicon tiled primers. Samples from MHV-1 infected mice were provided by Dr. Richard Bowen at Colorado State University. The MHV-1 strain used to infect the mice was obtained from American Type Culture Collection (Manassas, VA) and viral stock was.....
Document: Nontarget Advances in Bioinformatics Table 4 : Number of nontarget amplicons predicted in a multiplex reaction of tiled primers for 10 kb amplicons. As in Table 3 , but for the multiplexes of the 10 kb-amplicon tiled primers. Samples from MHV-1 infected mice were provided by Dr. Richard Bowen at Colorado State University. The MHV-1 strain used to infect the mice was obtained from American Type Culture Collection (Manassas, VA) and viral stock was propagated in murine fibroblast 17Cl-1 cells then used to infect C3H mice via intranasal route. Mice were sacrificed four days after inoculation and bronchoalveolar lavage (BAL) fluid was collected. RNA was extracted from the BAL samples using Invitrogen TRIZOL reagent, as per the manufacturer's instructions. RNA was converted to cDNA using Superscript III (Invitrogen) and random hexamers according to the manufacturer's protocol.
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