Selected article for: "catalytic activity and RT polymerase"
Author: Ross D. Overacker; Somdev Banerjee; George F. Neuhaus; Selena Milicevic Sephton; Alexander Herrmann; James A. Strother; Ruth Brack-Werner; Paul R. Blakemore; Sandra Loesgen
Title: Biological Evaluation of Molecules of the azaBINOL Class as Antiviral Agents: Specific Inhibition of HIV-1 RNase H Activity by 7-Isopropoxy-8-(naphth-1-yl)quinoline
  • Document date: 2019_1_23
    • ID: m2zw8eq4_19
    Snippet: However, since RNase H activity, the second catalytic activity of the HIV-1 reverse 223 transcriptase, is not detected in the above RT-polymerase assay, we investigated the effect of 224 B#24 on RNase H activity using a previously reported FRET based approach. 40, 41 Here, we used 225 a pair of oligonucleosides including an 18-mer strand of RNA containing a 3'-fluorescein 226 modification and an 18-mer strand of DNA with a 5'-dabcyl quencher modi.....
    Document: However, since RNase H activity, the second catalytic activity of the HIV-1 reverse 223 transcriptase, is not detected in the above RT-polymerase assay, we investigated the effect of 224 B#24 on RNase H activity using a previously reported FRET based approach. 40, 41 Here, we used 225 a pair of oligonucleosides including an 18-mer strand of RNA containing a 3'-fluorescein 226 modification and an 18-mer strand of DNA with a 5'-dabcyl quencher modification. When RNA 227 is cleaved from the RNA/DNA hybrid by RNase H activity, the fluorescent probe is removed 228 from its quenching partner (dabcyl) resulting in fluorescence. We found that B#24 inhibited 229

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