Selected article for: "plaque assay and serum antibody"
Author: Kim, Eun; Erdos, Geza; Huang, Shaohua; Kenniston, Thomas; Falo, Louis D.; Gambotto, Andrea
Title: Preventative Vaccines for Zika Virus Outbreak: Preliminary Evaluation
  • Document date: 2016_10_3
    • ID: jzcyxjxt_8
    Snippet: Six-to eight-week-old C57BL/6 female mice (five animals per group) were inoculated subcutaneously (s.c.) with 1 × 10 11 viral particles (v.p.) of Ad5.ZIKV-Efl or PBS as a negative control, and intradermally (i.d.) with MNA coated with 20 μg of ZIKV-rEfl. Two weeks after the primary immunization, mice were boosted intranasally (i.n.) or i.d. with the same dose of the respective immunogens. Mice were bled from the retro-orbital sinus at week 0, 2.....
    Document: Six-to eight-week-old C57BL/6 female mice (five animals per group) were inoculated subcutaneously (s.c.) with 1 × 10 11 viral particles (v.p.) of Ad5.ZIKV-Efl or PBS as a negative control, and intradermally (i.d.) with MNA coated with 20 μg of ZIKV-rEfl. Two weeks after the primary immunization, mice were boosted intranasally (i.n.) or i.d. with the same dose of the respective immunogens. Mice were bled from the retro-orbital sinus at week 0, 2, 4, and 6, and serum samples were evaluated for ZIKV antibody by enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization assay (PRNT). For the immunization study, a protocol approved by the University of Pittsburgh Institutional Animal Care and Use Committee was followed.

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