Selected article for: "dapi staining and fluorescence microscopy"
Author: Dong, Xiaoxv; Ni, Boran; Fu, Jing; Yin, Xingbin; You, Longtai; Leng, Xin; Liang, Xiao; Ni, Jian
Title: Emodin induces apoptosis in human hepatocellular carcinoma HepaRG cells via the mitochondrial caspase-dependent pathway
  • Document date: 2018_8_2
    • ID: 02r8i5n1_6
    Snippet: DAPI staining. The occurrence of apoptosis was evaluated by DAPI staining. Cells (4.0x10 5 cells/well) were seeded and incubated with different concentrations of emodin for 24 h. Following treatment, the cells were washed with PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich) for 15 min at room temperature, and then stained in the dark with a DAPI solution for 10 min at room temperature. Thereafter, the cells were washed with PBS and photogr.....
    Document: DAPI staining. The occurrence of apoptosis was evaluated by DAPI staining. Cells (4.0x10 5 cells/well) were seeded and incubated with different concentrations of emodin for 24 h. Following treatment, the cells were washed with PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich) for 15 min at room temperature, and then stained in the dark with a DAPI solution for 10 min at room temperature. Thereafter, the cells were washed with PBS and photographed under an inverted fluorescence microscopy (Olympus IX71; Olympus, Tokyo, Japan).

    Search related documents:
    Co phrase search for related documents
    • dapi staining and fluorescence microscopy: 1, 2, 3
    • dapi staining and room temperature: 1, 2, 3, 4, 5, 6, 7, 8
    • dapi staining and room temperature 10 min: 1
    • different concentration and fluorescence microscopy: 1
    • different concentration and room temperature: 1, 2, 3
    • fluorescence microscopy and room temperature: 1, 2, 3, 4, 5, 6, 7
    • fluorescence microscopy and room temperature 10 min: 1, 2
    • PBS wash and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • PBS wash and room temperature 10 min: 1