Author: Bancroft, Tara; DeBuysscher, Blair L.; Weidle, Connor; Schwartz, Allison; Wall, Abigail; Gray, Matthew D.; Feng, Junli; Steach, Holly R.; Fitzpatrick, Kristin S.; Gewe, Mesfin M.; Skog, Patrick D.; Doyle-Cooper, Colleen; Ota, Takayuki; Strong, Roland K.; Nemazee, David; Pancera, Marie; Stamatatos, Leonidas; McGuire, Andrew T.; Taylor, Justin J.
Title: Detection and activation of HIV broadly neutralizing antibody precursor B cells using anti-idiotypes Document date: 2019_10_7
ID: 63yvpuqx_28
Snippet: In the course of our sequencing experiments, we screened through the equivalent of 30 million B cells and only identified a few CDRH3 sequences recognized by the anti-idiotypes that showed >50% amino acid identity to iglb12. It is conceivable that the antiidiotypes were able to recognize rare CDRH3 sequences with higher identity to iglb12 but that we were unable to identify them due to limitations in the efficiency of cell sorting and BCR sequenc.....
Document: In the course of our sequencing experiments, we screened through the equivalent of 30 million B cells and only identified a few CDRH3 sequences recognized by the anti-idiotypes that showed >50% amino acid identity to iglb12. It is conceivable that the antiidiotypes were able to recognize rare CDRH3 sequences with higher identity to iglb12 but that we were unable to identify them due to limitations in the efficiency of cell sorting and BCR sequencing. The ability to screen a larger number of cells currently exceeds our capacity but is obtainable using enrichment and cell sorting in combination with droplet-based BCR sequencing approaches.
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