Author: Folarin, Onikepe A.; Ehichioya, Deborah; Schaffner, Stephen F.; Winnicki, Sarah M.; Wohl, Shirlee; Eromon, Philomena; West, Kendra L.; Gladden-Young, Adrianne; Oyejide, Nicholas E.; Matranga, Christian B.; Deme, Awa Bineta; James, Ayorinde; Tomkins-Tinch, Christopher; Onyewurunwa, Kenneth; Ladner, Jason T.; Palacios, Gustavo; Nosamiefan, Iguosadolo; Andersen, Kristian G.; Omilabu, Sunday; Park, Daniel J.; Yozwiak, Nathan L.; Nasidi, Abdusallam; Garry, Robert F.; Tomori, Oyewale; Sabeti, Pardis C.; Happi, Christian T.
Title: Ebola Virus Epidemiology and Evolution in Nigeria Document date: 2016_10_15
ID: 2g9ggwog_20
Snippet: DNA was depleted from the RNA samples using TURBO DNase (Ambion), and host ribosomal RNA was then depleted from the samples using an RNase H selective depletion method described elsewhere [2, 9, 10] . Complementary DNA was then synthesized from the resulting depleted RNA, Nextera XT libraries were constructed, and Illumina sequencing was carried out according to methods described elsewhere [2, 11] , with the modification that Nextera libraries we.....
Document: DNA was depleted from the RNA samples using TURBO DNase (Ambion), and host ribosomal RNA was then depleted from the samples using an RNase H selective depletion method described elsewhere [2, 9, 10] . Complementary DNA was then synthesized from the resulting depleted RNA, Nextera XT libraries were constructed, and Illumina sequencing was carried out according to methods described elsewhere [2, 11] , with the modification that Nextera libraries were generated using 16-18 cycles of PCR. Samples were sequenced on the MiSeq platform at RUN, and on both the MiSeq and HiSeq 2500 platforms (Illumina) at the Broad Institute.
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