Author: Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee
Title: Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus Document date: 2019_7_9
ID: 2hxlx1j2_14
Snippet: The sequences of 49 fully sequenced S genes and 39 complete genomes of global PEDV isolates were independently used in sequence alignments and phylogenetic analyses. Multiple sequence alignments were generated using the ClustalX 2.0 program [27] and the percentages of nucleotide sequence divergences were assessed using the same software. Phylogenetic trees were constructed from the aligned nucleotide or amino acid sequences using the neighbor-joi.....
Document: The sequences of 49 fully sequenced S genes and 39 complete genomes of global PEDV isolates were independently used in sequence alignments and phylogenetic analyses. Multiple sequence alignments were generated using the ClustalX 2.0 program [27] and the percentages of nucleotide sequence divergences were assessed using the same software. Phylogenetic trees were constructed from the aligned nucleotide or amino acid sequences using the neighbor-joining method and subsequently subjected to bootstrap analysis with 1000 replicates to determine the percentage reliability values of each internal node of the tree [28] . All phylogenetic trees were generated using Mega 4.0 software [29] .
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