Selected article for: "IPA strain and Ov IPA strain"
Author: OHTANI, Akifumi; KUBO, Masahito; SHIMODA, Hiroshi; OHYA, Kenji; IRIBE, Tadashi; OHISHI, Daiki; ENDOH, Daiji; OMATSU, Tsutomu; MIZUTANI, Tetsuya; FUKUSHI, Hideto; MAEDA, Ken
Title: Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea
  • Document date: 2015_2_27
    • ID: 4itsd2aq_14
    Snippet: Identification of isolates by PCR, sequence and phylogenetic analysis: Two isolates (22-58 and 24-100) were PCR positive when genus-specific and species-specific omp1 genes of C. pecorum were targeted. Nucleotide sequences of genus-specific PCR products were determined and compared with those of published C. pecorum omp1 genes. Omp1 gene partial sequence of the 2 isolates was 100% identical and showed the highest identity (99.8%) to the Bo/Yokoha.....
    Document: Identification of isolates by PCR, sequence and phylogenetic analysis: Two isolates (22-58 and 24-100) were PCR positive when genus-specific and species-specific omp1 genes of C. pecorum were targeted. Nucleotide sequences of genus-specific PCR products were determined and compared with those of published C. pecorum omp1 genes. Omp1 gene partial sequence of the 2 isolates was 100% identical and showed the highest identity (99.8%) to the Bo/Yokohama strain isolated from cattle with enteritis in Japan. Nucleotide similarities of the omp1 gene between the isolates and other published C. pecorum isolates were 89.2%, 85.2% and 82.0% for Bo/Maeda strain which was isolated from cattle with pneumonia in Japan, Ov/IPA strain which was isolated from sheep with polyarthritis in United States, and 66P130 strain which was isolated from cattle with enteritis in United States, respectively (Fig. 2, Table 1 ).

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