Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_510
Snippet: The DNA samples utilized in the study had been extracted from fleas infesting 96 cats in previously published Bartonella spp., hemoplasmas, and Rickettsia felis studies. They were maintained at À80°C until the present study. The fleas were collected from cats in eastern Australia (86) and the southern United States (4 Alabama, 6 Florida) and pooled in groups comprised of a maximum of 5 fleas before DNA extraction. A previously reported conventi.....
Document: The DNA samples utilized in the study had been extracted from fleas infesting 96 cats in previously published Bartonella spp., hemoplasmas, and Rickettsia felis studies. They were maintained at À80°C until the present study. The fleas were collected from cats in eastern Australia (86) and the southern United States (4 Alabama, 6 Florida) and pooled in groups comprised of a maximum of 5 fleas before DNA extraction. A previously reported conventional PCR assay utilizing primers pairs targeting the IS-1111 (IS-5, IS-9, IS-14 and IS-20) insertion sequences transposase elements in the C. burnetii genome was used. If positive amplicon was detected, genetic sequencing would be performed to confirm the presence of the pathogen.
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