Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_518
Snippet: During planned community outreach sterilization and vaccination clinics for local dogs with consenting owners, samples of ectoparasites were collected from infested dogs and preserved in alcohol for identification, and blood samples were collected in EDTA and non-additive tubes retained for testing. Sera were assayed for antigen of Dirofilaria immitis and antibodies against Ehrlichia canis/E. ewingii, Anaplasma phagocytophilum/A. platys, and Borr.....
Document: During planned community outreach sterilization and vaccination clinics for local dogs with consenting owners, samples of ectoparasites were collected from infested dogs and preserved in alcohol for identification, and blood samples were collected in EDTA and non-additive tubes retained for testing. Sera were assayed for antigen of Dirofilaria immitis and antibodies against Ehrlichia canis/E. ewingii, Anaplasma phagocytophilum/A. platys, and Borrelia burgdorferi using a commercial kit (SNAPÃ’ 4DXPlusÃ’, IDEXX Laboratories). Sera were also assayed for antibodies against Rickettsia species by indirect fluorescent antibody (IFA) testing (R. rickettsia antigen source). Total DNA was extracted from blood and evaluated with conventional polymerase chain reaction (PCR) assays to amplify DNA of Anaplasma species, Babesia species, Bartonella species, Ehrlichia species, Rickettsia species, and hemotropic Mycoplasma species (Hemoplasmas). Positive amplicons were sequenced to ascertain the species.
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