Author: Swatek, Kirby N.; Aumayr, Martina; Pruneda, Jonathan N.; Visser, Linda J.; Berryman, Stephen; Kueck, Anja F.; Geurink, Paul P.; Ovaa, Huib; van Kuppeveld, Frank J. M.; Tuthill, Tobias J.; Skern, Tim; Komander, David
Title: Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies Document date: 2018_3_6
ID: 3s86w4iw_15
Snippet: To further visualize Lb pro activity, we exploited a previously developed antibody that recognizes GlyGly-modified Lys residues (27, 28) . This anti-GlyGly antibody has so far only been used to enrich ubiquitinated tryptic peptides in MS applications (27, 28) . Rewardingly, probing these same reactions with the anti-GlyGly antibody produced a smear of strong signals across a wide range of molecular weights in the Lb pro -treated sample (Fig. 4A .....
Document: To further visualize Lb pro activity, we exploited a previously developed antibody that recognizes GlyGly-modified Lys residues (27, 28) . This anti-GlyGly antibody has so far only been used to enrich ubiquitinated tryptic peptides in MS applications (27, 28) . Rewardingly, probing these same reactions with the anti-GlyGly antibody produced a smear of strong signals across a wide range of molecular weights in the Lb pro -treated sample (Fig. 4A ). These signals correspond to the Lb pro -produced GlyGlymodified proteome.
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