Author: Strating, Jeroen R.P.M.; van der Linden, Lonneke; Albulescu, Lucian; Bigay, Joëlle; Arita, Minetaro; Delang, Leen; Leyssen, Pieter; van der Schaar, Hilde M.; Lanke, Kjerstin H.W.; Thibaut, Hendrik Jan; Ulferts, Rachel; Drin, Guillaume; Schlinck, Nina; Wubbolts, Richard W.; Sever, Navdar; Head, Sarah A.; Liu, Jun O.; Beachy, Philip A.; De Matteis, Maria A.; Shair, Matthew D.; Olkkonen, Vesa M.; Neyts, Johan; van Kuppeveld, Frank J.M.
Title: ITRACONAZOLE INHIBITS ENTEROVIRUS REPLICATION BY TARGETING THE OXYSTEROL-BINDING PROTEIN Document date: 2015_1_29
ID: 3kmqy07w_7
Snippet: Briefly, cells were seeded at a density of 5 x 10 3 cells per well in a 96-well cell culture plate in complete DMEM. Following incubation of 24 hours at 37°C, serial dilutions of the test compounds in complete DMEM were added in a total volume of 100 μL. Replicon RNA levels after 3 days of incubation were determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Primers used for detection of HCV replicon RNA were: 5'.....
Document: Briefly, cells were seeded at a density of 5 x 10 3 cells per well in a 96-well cell culture plate in complete DMEM. Following incubation of 24 hours at 37°C, serial dilutions of the test compounds in complete DMEM were added in a total volume of 100 μL. Replicon RNA levels after 3 days of incubation were determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Primers used for detection of HCV replicon RNA were: 5'-CCGGCTACCTGCCCATTC-3' (forward primer), 5'-CCAGATCATCCTGATCGACAAG-3' (reverse primer) and 5'-FAM-ACATCGCATCGAGCGAGCACGTAC-TAMRA-3' (probe).
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