Selected article for: "polyclonal antibody and slight modification"

Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin
  • Document date: 2015_12_17
  • ID: 5i84nomn_12
    Snippet: The cell fusion protocol was based on a previously described standard method [27] , with slight modification. The mouse that had the highest polyclonal antibody titer was sacrificed, after which spleen cells were mixed with SP2/O myeloma cells using polyethylene glycol 1500 (Roche, Germany). The ratio of spleen cells from the immunized mouse fused with SP2/O myeloma cells was about 5 : 1. Fused cells were grown in HAT selection medium, plated in .....
    Document: The cell fusion protocol was based on a previously described standard method [27] , with slight modification. The mouse that had the highest polyclonal antibody titer was sacrificed, after which spleen cells were mixed with SP2/O myeloma cells using polyethylene glycol 1500 (Roche, Germany). The ratio of spleen cells from the immunized mouse fused with SP2/O myeloma cells was about 5 : 1. Fused cells were grown in HAT selection medium, plated in 96-well microculture plates, and hybridoma supernatants were then screened for specific antibodies using indirect competitive ELISA.

    Search related documents:
    Co phrase search for related documents
    • antibody titer and hat selection medium: 1
    • antibody titer and high polyclonal antibody titer: 1, 2
    • antibody titer and myeloma cell: 1
    • antibody titer and polyclonal antibody titer: 1, 2, 3
    • antibody titer and selection medium: 1
    • antibody titer and specific antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
    • antibody titer and standard method: 1, 2
    • cell fusion and fuse cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • cell fusion and hat selection medium: 1
    • cell fusion and myeloma cell: 1, 2, 3, 4, 5
    • cell fusion and selection medium: 1, 2
    • cell fusion and specific antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • cell fusion and spleen cell: 1, 2
    • cell fusion protocol and myeloma cell: 1
    • cell fusion protocol and specific antibody: 1
    • cell fusion protocol and spleen cell: 1
    • hat selection medium and selection medium: 1, 2
    • hat selection medium and spleen cell: 1
    • high polyclonal antibody titer and polyclonal antibody titer: 1, 2