Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_12
Snippet: The cell fusion protocol was based on a previously described standard method [27] , with slight modification. The mouse that had the highest polyclonal antibody titer was sacrificed, after which spleen cells were mixed with SP2/O myeloma cells using polyethylene glycol 1500 (Roche, Germany). The ratio of spleen cells from the immunized mouse fused with SP2/O myeloma cells was about 5 : 1. Fused cells were grown in HAT selection medium, plated in .....
Document: The cell fusion protocol was based on a previously described standard method [27] , with slight modification. The mouse that had the highest polyclonal antibody titer was sacrificed, after which spleen cells were mixed with SP2/O myeloma cells using polyethylene glycol 1500 (Roche, Germany). The ratio of spleen cells from the immunized mouse fused with SP2/O myeloma cells was about 5 : 1. Fused cells were grown in HAT selection medium, plated in 96-well microculture plates, and hybridoma supernatants were then screened for specific antibodies using indirect competitive ELISA.
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