Author: Xin Zeng; Lingfang Li; Jing Lin; Xinlei Li; Bin Liu; Yang Kong; Shunze Zeng; Jianhua Du; Huahong Xiao; Tao Zhang; Shelin Zhang; Jianghai Liu
Title: Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy Document date: 2020_4_20
ID: 4w6caxwu_19
Snippet: Recombinant human ACE2-His (5μg/ml, 100μl per well) was coated on 96-well Maxisorp plates, followed by a pre-incubated mixture of the anti-RBD antibody titrated into a constant amount of RBD-mFc (1µg/ml). RBD binding to ACE2 was detected using HRP conjugated anti-mouse Fc antibody......
Document: Recombinant human ACE2-His (5μg/ml, 100μl per well) was coated on 96-well Maxisorp plates, followed by a pre-incubated mixture of the anti-RBD antibody titrated into a constant amount of RBD-mFc (1µg/ml). RBD binding to ACE2 was detected using HRP conjugated anti-mouse Fc antibody.
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