Selected article for: "imagej software and primary antibody"
Author: Jorge Blazquez-Prieto; Covadonga Huidobro; Ines Lopez-Alonso; Laura Amado-Rodriguez; Paula Martin-Vicente; Cecilia Lopez-Martinez; Irene Crespo; Cristina Pantoja; Pablo J Fernandez-Marcos; Manuel Serrano; Jacob I Sznajder; Guillermo M Albaiceta
Title: Cellular senescence limits acute lung injury induced by mechanical ventilation
  • Document date: 2020_3_25
    • ID: ebwxryai_21
    Snippet: For immunofluorescence studies, slides were deparaffinated and antigens retrieved in citrate buffer 0.1M (pH=9). The autofluorescence of the tissue was diminished using a Sudan black B solution and sections were permeabilized (0.1% Triton X-100 in PBS for 15 minutes), blocked (1% BSA in PBS) and incubated overnight at 4°C with the primary antibody (Supplementary Table 3 ). After 24 hours, the slices were incubated with the corresponding secondar.....
    Document: For immunofluorescence studies, slides were deparaffinated and antigens retrieved in citrate buffer 0.1M (pH=9). The autofluorescence of the tissue was diminished using a Sudan black B solution and sections were permeabilized (0.1% Triton X-100 in PBS for 15 minutes), blocked (1% BSA in PBS) and incubated overnight at 4°C with the primary antibody (Supplementary Table 3 ). After 24 hours, the slices were incubated with the corresponding secondary fluorescent antibody at room temperature for 1 hour. Images were taken using a confocal microscopy (Leica SP8) at 400x and 630x. The number of positive and negative nuclei were automatically quantified using ImageJ software (NIH, USA).

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