Author: Lee, Charlie Wah Heng; Koh, Chee Wee; Chan, Yang Sun; Aw, Pauline Poh Kim; Loh, Kuan Hon; Han, Bing Ling; Thien, Pei Ling; Nai, Geraldine Yi Wen; Hibberd, Martin L.; Wong, Christopher W.; Sung, Wing-Kin
Title: Large-scale evolutionary surveillance of the 2009 H1N1 influenza A virus using resequencing arrays Document date: 2010_2_25
ID: 1rhy8td0_39
Snippet: The second-step processes the ambiguous bases. For bases confirmed to be mutations through NHIP analysis and satisfy the substitution bias rule, they are graded as high quality sequence calls and denoted in the FASTA sequence as UPPER CASE characters. For bases confirmed to be an isolated error through NHIP analysis and also satisfy the substitution bias rule, they are graded as low confidence sequence calls and denoted in the FASTA sequence in l.....
Document: The second-step processes the ambiguous bases. For bases confirmed to be mutations through NHIP analysis and satisfy the substitution bias rule, they are graded as high quality sequence calls and denoted in the FASTA sequence as UPPER CASE characters. For bases confirmed to be an isolated error through NHIP analysis and also satisfy the substitution bias rule, they are graded as low confidence sequence calls and denoted in the FASTA sequence in lower case characters. The rest of the bases are called 'N' in the FASTA sequence. The flowchart of EvolSTAR is shown in Figure 1 .
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